July 1, 2024
By dev@agencyjet.com

Western blot Resources

Western blot remains one of the most common techniques to detect and identify proteins from cell lysates or tissue homogenates.
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Western blot Products for Every Step of the Journey

Western blot remains one of the most common techniques to detect and identify proteins from cell lysates or tissue homogenates. Exreprotein offers a wide selection of gels, molecular weight markers, buffers and antibodies to save time setting up experiments and get reliable data.

Fast Gel Prep in 20 minutes. Run Your Gel in 30 minutes

Exreprotein EXREsolve FlexKD PAGE Gel kits are optimized to make gel preparation a breeze. The necessary reagents are included in the kit.

✓ 20 minute gel preparation time. Stacking gel can be poured while separation gel is setting.

✓ 30 minute electrophoresis time. Safely use high voltage up to 300V to resolve proteins faster.

✓ Sharp and consistent bands.

✓ Safer handling. No TEMED is required or used in the formulation.

✓ Save money. More cost-effective than other alternatives.

✓ Options for all protein molecular weights.

See more information on EXREsolve FlexKD kits

Precast Gels. Ultimate Convenience When Results Matter

Our EXREsolveTM Precast Gels are manufactured for consistent results allowing you to focus on running your gels without worrying about prepping and pouring your own gels. ✓ Easy to use. Leave the gel casting to us. ✓ 30 minute electrophoresis time. Safely use high voltage up to 300V to resolve proteins faster. ✓ Sharp and consistent bands. ✓ Save money. More cost-effective than other suppliers. ✓ The buffer is included. Two packs of MOPS-SDS buffer powder included. Quick prep time. ✓ Safer handling. No TEMED is required or used in the formulation ✓ Gel options for your protein size:
    • ✓ 12% gel: 10-160 kDa
    • ✓ 4-20% gel: 10-250 kDa
    • ✓ 4-12% gel: 20-300 kDa
Browse the EXREsolve Precast Gel Kits

Prestained Protein Molecular Weight Standards

Our prestained protein molecular weight standards help visualize the correct molecular weight for your proteins of interest.

✓ Multiple colors visually identify bands of different sizes

✓ Ready to use. Load directly. No heating, dilution or reducing agent required.

✓ Easily observe electrophoresis progress

✓ Visually verify transfer between gel and membrane.

✓ Convenient ladder ranges for your protein targets:

See more information

Visualize Your Results

Whether you need to stain total protein or use HRP-linked antibodies for chemiluminescent detection, we have you covered.

✓ EXREsolve Blue Protein Staining Reagent. Total protein staining Coomassie alternative.

✓ EXREsolve ECL Chemiluminescent Substrate.

    • ✓ Improved sensitivity. 10x better than average ECL reagent
    • ✓ Low background
    • ✓ Stable
Learn more

Simplify Your Lab Prep. Easy to use buffers.

Keep your buffer prep simple and consistent. Choose from our ready-made buffer or instant buffer powders:

✓ Instant HEPES Buffer

✓ Instant Membrane Transfer Buffer

✓ Instant MES Buffer

✓ Instant MOPS Buffer

✓ Instant PBS Buffer

✓ Instant PBS-T Buffer

✓ InstantTAE Buffer

✓ Instant TBS Buffer

✓ Instant TBS-T Buffer

✓ Instant TBS-T Blocking Buffer

✓ Instant Tris-Glycine Buffer

✓ Instant Tris-Glycine-SDS Buffer

Browse All Buffers

Frequently Asked Questions (FAQ)

1. What are the advantages of using a Precast SDS-PAGE gel instead of casting my own gel?
Precast SDS-PAGE gels provide consistent polymerization, uniform pore structure, and improved reproducibility while reducing preparation time and minimizing experimental variability.
2. Why are prestained protein molecular weight markers important?
Prestained protein molecular weight markers allow real-time tracking during electrophoresis and help verify efficient protein transfer during Western blot analysis.
3. What is the purpose of a protein staining solution after electrophoresis?
Protein staining solutions visualize total protein in gels, allowing confirmation of protein loading, expression levels, and purification efficiency.
4. How do ECL reagents work in Western blot detection?
ECL reagents react with HRP-conjugated antibodies to produce a chemiluminescent signal, enabling sensitive detection of target proteins on membranes.
5. How can I improve signal sensitivity in my Western blot?
Use high-quality Precast SDS-PAGE gels, validated prestained protein molecular weight markers, optimized blocking conditions, and sensitive ECL reagents to maximize signal intensity while minimizing background noise.
6. What is the buffer composition for electrophoresis buffers like TGS, MES, MOPS, and TAE?
TGS contains Tris, glycine, and SDS for protein SDS-PAGE. MES and MOPS are sulfonic acid-based buffers optimized for different protein molecular weight ranges. TAE contains Tris, acetate, and EDTA and is commonly used for DNA agarose gel electrophoresis.
7. What is the standard PBS buffer composition?
Our PBS buffer contains 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, and 1.8 mM KH₂PO₄ at pH 7.4.
8. Are these reagents suitable for sensitive cell culture work?
Yes. All reagents are sterile, endotoxin-controlled, and optimized for biological compatibility.
9. What is HEPES buffer used for?
HEPES buffer maintains stable physiological pH in cell culture and biochemical assays, especially outside CO₂ incubators.
10. Why are low endotoxin levels important in laboratory reagents?
Low endotoxin buffers and reagents are essential for sensitive cell culture systems and therapeutic protein workflows to prevent immune activation and variability.
11. Why choose ready-to-use buffers instead of preparing them in-house?
Ready-to-use laboratory reagents reduce preparation time, minimize variability, improve reproducibility, and ensure consistent experimental performance.
12. Do you support ready to use electrophoresis or western blot related buffer?
Yes. We provide Membrane Transfer, TBST-Blocking buffer, Tris-Glycin-SDS, MOPS, etc.
13. How do research-grade reagents improve reproducibility?
High purity, strict quality control, and lot-to-lot consistency minimize variability, ensuring reliable and repeatable experimental results.
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