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EXact-Cutโข DpnI Restriction Endonuclease
Catalog: EXNA011
$49.00
Description
EXact-Cut DpnI Restriction Endonuclease (EXNA011) selectively cuts methylated Gm6ATC sequences in dam+ bacterial DNA. As part of the EXact-Cut series, it works in a single universal buffer. This design then speeds up digestion to 5โ15 minutes. Furthermore, it is widely used in site-directed mutagenesis workflows to remove template plasmid DNA.
Key Features
โ Reduced star activity
โ Save time. Digest DNA in 5-15 minutes
โ All Exact-Cutโข Enzymes 100% active in a single buffer (included)
โ Purple Loading Dye included
Size
โ 500 Units
Details
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EXact-Cutโข DpnI Restriction Endonuclease
For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Restriction Enzyme Site | 5สนโฆG Am6โT Cโฆ3สน 3สนโฆC T โAm6Gโฆ5สน Isoschizomers: MalI (Isoschizomers may have different methylation sensitivities) |
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1 ug of ฮป DNA in 1 hour at 37ยฐC in a total reaction volume of 50 uL. |
| Recommended Reaction Conditions | 1 x EXact-Cutโข Buffer Incubate at 37ยฐC Refer to datasheet for reaction setup |
| Heat Inactivation | 1. Incubate at 80ยฐC for 20 minutes 2. Add appropriate volume of 6X Gel Loading Dye, according to the reaction system |
| Product Components | EXact-Cutโข DpnI (10 Units/uL): 500 Units EXact-Cutโข 10X Buffer: 1 mL 6X Gel Loading Dye, Purple: 1 mL |
Storage & Preparation
| Shipping | Shipped on blue ice. |
| Stability & Storage | Store at -20ยฐC for up to 24 months. |
Data Images
Background
| Product Summary | EXact-Cutโข DpnI Restriction Endonuclease is engineered for high specificity, reduced star activity, and time saving DNA digestion in 5-15 minutes. To simplify experimental design using multiple restriction enzymes, our entire range of EXact-Cutโข restriction endonucleases are 100% active in our EXact-Cutโข buffer (included) and are optimized for single-tube reactions along with digestion and ligation protocols. |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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