Elisa Kit Resources

Sandwich-based enzyme-linked immunoassays centered around matched antibody pairs tested for specificity and reproducibility.

Complete ELISA Kits to Get Results Quickly and Accurately

Sandwich-based enzyme-linked immunoassays centered around tested matched antibody pairs for specificity and reproducibility. Our ELISA kits contain precoated plates and all the reagents required to perform an ELISA. Our ELISA kits are suitable for serum, plasma and cell culture supernates.

✓ Quick assay time. Less than 3 hours. Optimized protocol for accurate results.

✓ Specificity for target protein. Selected matched antibody pairs.

✓ High reproducibility. Low % CVs.

✓ Sensitivity in low pg/mL.

ELISA Kits for Your Research Area

We offer ELISA kits for human, mouse and rat samples from a wide range of cytokine and growth factor families.

Interleukins: IL-1 beta, IL-2, IL-4, IL-6, IL-10 and more
Interferons: IFN-alpha and IFN-gamma
Growth Factors: EGF, HGF, TGF-beta 1, FGF-basic, VEGF and more
Chemokines: MCP-1/CCL2, IP-10/CXCL10, TARC/CCL17 and more
Additional Biomarkers: Adiponectin, C-Reactive Protein (CRP), Granzyme B, Resistin, TNF-alpha and more

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ELISpot Kits for Single-Cell Detection

ELISpot Kits are widely used to detect analyte-secreting cells at the single-cell level. This gives ELISpot assays a unique advantage in measuring the antigen-specific immune response of immune cells and subtypes. ELISpots remain an important tool in vaccine research in terms of detecting T cell response.

✓ Ready-to-use kit.

✓ Kit includes: precoated PVDF plate, detection antibody, Streptavidin-HRP, AEC color development system, dilution buffer, wash buffer and cell stimulation reagent.

✓ You add your cells and design the experimental cell culture conditions in the ELISpot wells.

✓ Analyte-secreting cells will be detected by precipitation of captured analyte on the PVDF membrane. These “spots” are quantitated to determine the presence of individual analyte-secreting cells.

ELISpot Kits for Your Research Area

We offer ELISpot kits for human and mouse cell detection for the following cytokines:

✓ Interleukins: IL-2, IL-4, IL-10 and IL-17A

✓ Interferons: IFN-gamma

✓ TNF-alpha

Browse ELISpot Kits

Example of IFN-gamma ELISpot detection of following stimulation with PMA and Ionomycin compared to no stimuli negative control (media only)

Frequently Asked Questions (FAQ)

Exreprotein LLC provides a comprehensive portfolio of assay kits, including ELISA test kits, ELISA diagnostic kits (RUO), ELISpot kits, and custom ELISA kits. Our products support cytokine detection, biomarker quantification, immune profiling, and cell function analysis for biomedical and life science research applications.

ELISA kits measure the total concentration of proteins, such as cytokines or growth factors, in bulk samples, such as serum or plasma. In contrast, ELISpot kits detect secreted proteins at the single-cell level, allowing researchers to quantify the number of cells actively producing a specific cytokine.
ELISA is ideal for quantitative protein measurement, while ELISpot is preferred for functional immune cell analysis and vaccine or T cell studies.

Absolutely. As an experienced ELISA kit manufacturer, we offer custom ELISA and ELISpot kits tailored to your specific biomarker or workflow. Services include antibody pair screening, assay optimization, recombinant standard production, and validation in your sample matrix. Custom solutions are ideal for novel proteins, engineered constructs, or specialized research assays.

Our ELISA diagnostic kits and ELISA test kits are supplied for Research Use Only (RUO). They are intended for laboratory and preclinical research applications and are not approved for clinical or diagnostic use in patients. For regulated clinical testing, additional validation and approvals would be required.