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High-Purity Recombinant Proteins for Biomedical Research

Exreprotein LLC delivers research-grade proteins and custom protein expression services built on advanced expression and purification technologies. Exreprotein LLC offers a growing portfolio of recombinant human cytokines and growth factors produced with verified biological activity and low endotoxin content for sensitive research applications.

From foundational reagents such as bovine serum albumin and Human Serum Albumin to specialized growth factors including recombinant human HGF protein, Human Noggin, TNF-alpha, GDNF, and PDGF-BB, every product is engineered for purity, low endotoxin content, and traceable quality.

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How We Evaluate Recombinant Protein Quality

Not all recombinant proteins meet the same quality standards. Proper evaluation is essential
for reproducible and biologically meaningful results.

  1. Purity and IdentityHigh-quality recombinant proteins should meet:
    • ≥97% purity by SDS-PAGE and/or HPLC
    • Confirmed identity by mass spectrometry or peptide mapping
  2. Endotoxin ContentEndotoxins can alter cell behavior and activate inflammatory pathways. Recommended limits include:
    • <0.1 EU/µg for general in vitro research
    • <0.01 EU/µg for highly sensitive cell-based assays
  3. Biological ActivityFunctional validation may include:
    • Receptor binding assays
    • Cell-based signaling or proliferation assays
    • Protein-specific functional readouts
  4. Optimized Lyophilization and Product TraceabilityExreprotein LLC proteins are commonly supplied in optimized lyophilized formats to ensure:
    • Long-term stability
    • Preserved biological activity
    • Consistent reconstitution
    • Simplified storage and shipping

Custom Protein Expression and Purification Services

In addition to catalog proteins, Exreprotein LLC provides custom protein expression services
using the same platforms applied to our research-grade products.

  1. Custom Expression Capabilities
    • Codon optimization and gene synthesis
    • Expression in bacterial, yeast, insect, or mammalian systems
    • Native or engineered constructs
    • Tag-free or cleavable-tag designs
  2. Custom Purification and Quality Targets
    • Multi-step purification strategies
    • Defined endotoxin specifications
    • Host cell protein and residual DNA clearance
    • Protein-specific formulation and lyophilization