EXREnu™ Human Serum-Free Lymphocyte Medium

EXREnu™ Human Serum-Free Lymphocyte Medium is optimized for high-density in vitro culture and expansion of human lymphocytes, cytokine-induced killer (CIK) cells, natural killer (NK) cells and dendritic cells from human peripheral blood mononuclear cells (PBMCs). This medium is serum-free and xeno-free to improve reproducibility and reduce risk of contaminating factors from earlier methods using feeder cells and serum supplements1. This chemically-defined medium contains albumin, transferrin, and insulin to support feeder cell-free and serum-free expansion of lymphocytes. Additional recombinant cytokines and activation methods are compatible with this medium for individually optimized growth and expansion of target lymphocyte populations.

EXREnu™ Human Serum-Free Lymphocyte Medium supports culture of lymphocytes at a density of 5×105 – 5×106 cells/mL in a static culture vessel at a recommended depth ≤ 1.5 cm to ensure good gas exchange. The medium also supports high-density culture of lymphocytes in a bioreactor, where conditions should be optimized by the requirements of each user. The addition of autologous plasma, human AB serum, FBS or serum substitutes can further improve the cell proliferation rate. The EXREnu™ Cell Culture Supplemental mix (Catalog #: EXCM008) is recommended to obtain the optimal culture result.