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For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
Key Features
✓ Detects endogenous Histone H3
✓ Western blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
✓ Monoclonal Mouse
Size
✓ 100 ug
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Source | Monoclonal Mouse |
| Specificity | Detects endogenous human, mouse, rat, and yeast Histone H3. |
| Immunogen | Recombinant Histone H3 protein |
| Purification | Antigen affinity chromatography |
| Formulation | PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide as a preservative and 50% glycerol. |
| Concentration | 1 mg/mL |
| Applications | Western blot: 1:2,000-5,000 recommended dilution Immunofluorescence: 1:100-500 recommended dilution Immunohistochemistry: 1:50-300 recommended dilution Immunoprecipitation: 1:200 recommended dilution For optimal performance, this reagent should be titrated for each application. |
Storage & Preparation
| Shipping | Shipped on blue ice. |
| Stability & Storage | 12 months from date of receipt at -15°C to -20°C. |
Data Images
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| Detection of Histone H3 by Western blot. Endogenous Histone H3 was detected at approximately 15 kDa in 1) HeLa cell lysates 2) RAW cell lysates, 3) mouse brain tissue, and 4) rat brain tissue using Catalog # EXAB014 at 1:5,000 dilution. |
Background
| Alternative Names | H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A |
| Tissue Specificity | Blood, Epithelium, Kidney, Lung, Ovary, Spleen, Uterus |
| Cellular Localization | Nucleus, Chromosome |
| UniProt | Human: P68431 Rat: Q6LED0 |
| Gene Symbol | H3C1 |
| Entrez Gene ID | Human: 8350 Mouse: 319152 Rat: 291159 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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