Recombinant DNA/RNA ExreNuclease™ (EXRP156)
$249.00 – $4,249.00
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Key Features for Recombinant DNA/RNA ExreNuclease™
✓ Endotoxin Level: <0.001 EU/1000 Units (<1 EU/1000 KU)
✓ Purity: >98-99% SDS-PAGE
✓ Biological/Enzymatic Activity: Yes
✓ Expression System: E. coli
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Biological Activity | Determined by its ability to cleave all single or double stranded DNA and RNA similar to the universal nuclease Benzonase®. The specific activity is typically >1.5×106 Unit per mg. |
| Purity | >98-99% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.001 EU per 1000 Units (< 1 EU/1000KU) of the protein as determined by the LAL method. |
| Expression System | E. coli |
| Accession Number | P13717.2 |
| Sequence | Met1-Asn266 |
| Molecular Weight | 28.9 kDa (monomer, predicted) |
| Formulation | PBS, pH 7.4, with 50% Glycerol and 2 mM MgCl2 |
Storage & Preparation
| Shipping | Shipped on blue ice. Store immediately at -20°C upon receipt. |
| Stability & Storage |
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Data Images
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| Activity Data. ExreNuclease at 0, 5.0, 1.0, and 0.1 Units was incubated with 36 μg of DNA/RNA mixture for 30 minutes at 37°C, then separated and visualized by staining in a 1.5% agarose gel. Lots #1 and 2 are before and after proprietary endotoxin removal procedure, respectively. | Purified ExreNuclease (Lane 1: 0.5 μg, Lane 2: 2.0 μg) on SDS-PAGE under reducing conditions. The gel was stained using Coomassie® Blue showing a single band at 29 kDa and purity greater than 98-99%. |
Background
| Alternative Names | Nuclease A, NucA |
| Function | Serratia marcescens Nuclease A (NucA) is a secreted, magnesium-dependent endonuclease that indiscriminately degrades both DNA and RNA, whether single- or double-stranded, linear, circular, or chromosomal, plasmid or viral, into into short 2–5-base oligonucleotides. Recombinant NucA, such as ExreNucleaseTM, has applications in bio-processing to meet guidelines for host cell DNA and viral DNA clearance, lowering lysate viscosity, improving clarification and chromatography, and cleaning up nucleic-acid contamination in cell-free assays. |
| Cellular Localization | Secreted enzyme |
| UniProt | P13717.2 |
| Gene Symbol | NucA |
Benzonase® is a registered trademark of Merck KGaA Corporation
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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