Recombinant Human Hyper IL-6 Protein (EXRP068)
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For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
Key Features
✓ Endotoxin Level: <0.005 EU per 1 ug
✓ Purity: >98% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: CHO cell line
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Biological Activity | This IL-6/IL-6R fusion protein stimulates proliferation in DS-1 cells (IL-6R+), albeit less than IL-6 alone. Also, IL-6/IL-6R fusion protein inhibits the proliferation of HUVECs (IL-6R-). Determined by a cell proliferation assay using DS-1 mouse lymphoblast cells, the ED50 for this effect is typically <750 pg/mL. Also determined by an inhibition of proliferation assay using HUVECs, the ED50 for this effect is typically 1-10 ng/mL. |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.005 EU/1ug of the protein as determined by the LAL method. |
| Expression System | CHO cell line. |
| Accession Number | P08887 (IL-6 R)Â & Â P05231 (IL-6) |
| Sequence |
sIL6R (Val112-Ala323)-GSL-IL6（Val30-Met212） VPPEEPQLSC FRKSPLSNVV CEWGPRSTPS LTTKAVLLVR KFQNSPAEDF QEPCQYSQES QKFSCQLAVP EGDSSFYIVS MCVASSVGSK FSKTQTFQGC GILQPDPPAN ITVTAVARNP RWLSVTWQDP HSWNSSFYRL RFELRYRAER SKTFTTWMVK DLQHHCVIHD AWSGLRHVVQ LRAQEEFGQG EWSEWSPEAM GTPWTESRSP PAGGGGSGGG GSVEPVPPGE DSKDVAAPHR QPLTSSERID KQIRYILDGI SALRKETCNK SNMCESSKEA LAENNLNLPK MAEKDGCFQS GFNEETCLVK IITGLLEFEV YLEYLQNRFE SSEEQARAVQ MSTKVLIQFL QKKAKNLDAI TTPDPTTNAS LLTKLQAQNQ WLQDMTTHLI LRSFKEFLQS SLRALRQM |
| Molecular Weight | 45.9 kDa (predicted), 42 kDa (observed on SDS-PAGE gel stained with Coomassie® Blue) |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. If desired, 5% Trehalose or 0.1% BSA may be added. |
Data Images
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| Recombinant Human Hyper IL-6 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue, showing a single band at 56-60 kDa and purity greater than 98%. | Determined by a cell proliferation assay using DS-1 mouse lymphoblast cells. The ED50 for this effect is typically <750 pg/mL. | Determined by an inhibition of proliferation assay using HUVECs. The ED50 for this effect is typically 1-10 ng/mL. |
Background
| Alternative Names | Interleukin 6, Interferon Beta-2, IFN-Beta-2, IFNB2, B-Cell Stimulatory Factor 2, BSF2, Hybridoma Growth Factor, HGF, HSF, CTL Differentiation Factor, CDF |
| Function | IL-6 plays a role in regulating immune response, inflammation, hematopoiesis, metabolism, and neurological processes. IL-6 influences the growth and differentiation of hematopoietic progenitor and stem cells. In the innate immune response, IL-6 is released by macrophages and dendritic cells. During the adaptive immune response, IL-6 plays a role in B cell maturation and differentiation of T cells into T follicular helper (Tfh) cells and Th17 cells. Muscle tissue and the central nervous system response to IL-6 has been linked to lipolysis and modulation of insulin response. IL-6 signaling occurs via binding to IL-6R alpha (CD126) and subsequent dimerization with the signal-transducing gp130 subunit. |
| Tissue Specificity | IL-6 expression has been reported in muscle tissue, endothelial tissue, lung, intestine, and adipose tissue. |
| Cellular Localization | Secreted protein |
| Involvement in Disease | Dysregulation of IL-6 is implicated in rheumatoid arthritis, systemic juvenile idiopathic arthritis (sJIA) inflammatory bowel disease (IBD), autoimmune hepatitis, atherosclerosis, insulin resistance, type 2 diabetes, fatty liver disease, obesity-related inflammation, Castleman disease, Behçet’s disease Alzheimer’s disease, systemic lupus erythematosus, multiple myeloma, and various cancers (prostate, breast, and lung) through actions on the tumor microenvironment. |
| UniProt | P08887 (IL-6 R)Â & Â P05231 (IL-6) |
| Gene Symbol | IL6R |
| Entrez Gene ID | 3569 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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