Recombinant Human Serum Albumin/HSA Protein (EXRP052)
Price range: $90.00 through $499.00
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For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
Key Features
✓ Endotoxin Level: <0.005 EU/1ug (<5 EU/mg)
✓ Purity: >98% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: HEK cell line, with no Fetal Bovine Serum (FBS)
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Biological Activity | The carrier effect of Recombinant Human Serum Albumin/HSA was determined by enhancement of cell proliferation using TF-1 human cells. Stout, et al. (2022) Commun Biol 5:466. Cell proliferation in TF-1 human cells was induced in a dose-dependent manner by either Recombinant Human GM-CSF or Recombinant Human IL-3 (Catalog # EXRP037) and demonstrated >25% enhancement in the presence of rHSA. |
| Purity | >98% under reducing conditions by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.005 EU/1ug (<1 EU/mg) of the protein as determined by the LAL method. |
| Expression System | HEK cell line, with no Fetal Bovine Serum (FBS). |
| Accession Number | NP_000468.1 |
| Sequence | Asp25-Leu609
DAHKSEVAHR FKDLGEENFK ALVLIAFAQY LQQCPFEDHV KLVNEVTEFA KTCVADESAE NCDKSLHTLF GDKLCTVATL RETYGEMADC |
| Molecular Weight | 66.5 kDa (monomer, predicted) observed on SDS-PAGE gel stained with stained using Coomassie® Blue under reducing conditions |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 1 mg/mL in sterile PBS. |
Data Images
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| Recombinant Bovine Human Albumin/HSA Protein (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 66 kDa and purity greater than 98%. | Carrier effect of rHSA is observed in the cell proliferation in TF-1 human cells treated with Recombinant Human IL-3 (Catalog # EXRP037) in the presence of 25 µg/mL rHSA. The carrier effect due to rHSA does not alter the ED50 in this assay |
Background
| Alternative Names | Albumin, HSA |
| Function | Human serum albumin (HSA) is a ~66.5 kDa, non-glycosylated plasma protein that contributes critically to physicochemical homeostasis in biochemical and cell culture systems through regulation of osmotic pressure and pH buffering capacity. HSA functions as a high-capacity carrier protein with multiple ligand-binding domains, enabling reversible binding and transport of a wide range of hydrophobic and amphipathic molecules, including long-chain fatty acids, steroid and thyroid hormones, bilirubin, and divalent metal ions. In addition to its transport functions, HSA exhibits protein-stabilizing activity by limiting denaturation, aggregation, and proteolytic degradation of labile proteins and enzymes. Owing to these combined buffering, carrier, and protective properties, recombinant HSA effectively recapitulates many of the essential biological functions of serum when used in chemically defined cell culture media. |
| Tissue Specificity | Plasma |
| Cellular Localization | Secreted protein |
| UniProt | A0A140T897 |
| Gene Symbol | ALB |
| Entrez Gene ID | 280717 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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