Recombinant Human TNF-alpha Protein Animal Component-Free (EXRP022-ACF)
Price range: $90.00 through $1,549.00
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For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
Key Features
✓ Endotoxin Level: <0.001 EU/1ug
✓ Purity: >98% by SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: E. coli using an animal component-free system
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Biological Activity | Determined by a cell proliferation assay using mouse L-929 cells treated with Actinomycin D. The ED50 for this effect is typically <40 pg/mL. |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Endotoxin | <0.001 EU per 1 μg of the protein as determined by the LAL method |
| Expression System | Mammalian cell line. |
| Accession Number | P01375.1 |
| Sequence | Val77-Leu233 VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL |
| Molecular Weight | 17.4 kDa (monomer, predicted) |
| Formulation | Lyophilized from sterile phosphate buffer with Trehalose, pH 8.0 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. Optionally, use PBS with 0.1% BSA (EXRP053). |
Data Images
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| Determined by a cell proliferation assay using mouse L-929 cells treated with Actinomycin D. The ED50 for this effect is typically <40 pg/mL. | HEK293 cells stably transfected with NF-κB-SEAP reporter respond to treatment with TNF-alpha (EXRP022). | Recombinant Human TNF-alpha (2 μg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at about 15 kDa and purity >98%. |
Background
| Alternative Names | Tumor Necrosis Factor alpha, TNF, TNFA, APC1, TNFSF2, Tumor Necrosis Factor Ligand Superfamily Member 2, Differentiation Inducing Factor, DIF, Cachectin |
| Function | Tumor Necrosis Factor-alpha (TNF-alpha) was initially associated as a proinflammatory cytokine expressed by macrophages. However, TNF-alpha also regulates apoptosis, tumor cell death, lipid metabolism and cell differentiation of T cells, B cells and NK cells. There are 2 receptors for TNF-alpha, a ubiquitously expressed TNFR1 (CD120a) homotrimer containing an apoptotic pathway trigger and the TNFR2 (CD120b) homotrimer that is commonly restricted to epithelial, endothelial and specific immune cells. |
| Tissue Specificity | TNF-alpha expression has been identified in adipose tissue, microglia, fibroblasts, endothelia tissue, and epithelial tissue. |
| Cellular Localization | Membrane-bound and cleaved soluble protein forms |
| Involvement in Disease | Dysregulation of TNF-alpha is implicated in rheumatoid arthritis, inflammatory bowel disease (IBD), psoriasis, ankylosing spondylitis, systemic lupus erythematosus (SLE), systemic sclerosis (SSc), multiple sclerosis (MS) and insulin resistance in type 2 diabetes |
| UniProt | P01375 |
| Gene Symbol | TNF |
| Entrez Gene ID | 7124 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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