Recombinant Mouse IFN-gamma Protein (EXRP038)
Price range: $90.00 through $1,129.00
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For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
Key Features
✓ Endotoxin Level: <0.02 EU/1ug
✓ Purity: >95% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: E. coli
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Biological Activity | Determined by the ability to inhibit proliferation of WEHI-279 mouse B cells. The ED50 for this effect is typically <0.2 ng/mL. |
| Purity | >95% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.02 EU per 1 µg of the protein as determined by the LAL method. |
| Expression System | E. coli |
| Accession Number | P01580 |
| Sequence | His23-Cys155, with an N-terminal Met
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC |
| Molecular Weight | 15.6 kDa (predicted) |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
Data Images
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| Determined by the ability to inhibit proliferation of WEHI-279 mouse B cells. The ED50 for this effect is typically <0.2 ng/mL. | Recombinant Mouse IFN-gamma (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 11 kDa and purity greater than 95%. |
Background
| Alternative Names | Interferon gamma, IFNG, Immune Interferon, IFG, IFI |
| Function | Interferon-gamma (IFN-gamma) is a protein with predominant roles in the innate and adaptive immune response. It is secreted by Th1 cells, cytotoxic T cells, macrophages, dendritic cells, mucosal epithelial cells and NK cells. IFN-gamma promotes the differentiation of T cells into Th1 cells. Th1 cells produce IFN-gamma themselves, creating a positive feedback loop that amplifies the immune response. In addition, IFN-gamma upregulates monocytes, macrophages, and dendritic cells while increasing MHC class I and II expression. B Cell immunoglobulin class switching is also influenced by IFN-gamma. IFN-gamma RI (CD119/IFNGR1) is the ligand-binding subunit that heterodimerizes with the IFN-gamma RII (IFNGR2). |
| Tissue Specificity | IFN-gamma expression has been identified in vascular endothelial cells, respiratory and gastrointestinal epithelial cells. |
| Cellular Localization | Secreted protein |
| Involvement in Disease | Dysregulation of IFN-gamma is associated with autoimmune disorders (multiple sclerosis (MS), rheumatoid arthritis (RA), type 1 diabetes, and inflammatory bowel diseases (IBD)), allergic asthma, atherosclerosis, hemophagocytic lymphohistiocytosis (HLH), Alzheimer’s disease, Parkinson’s disease and multiple sclerosis (MS). |
| UniProt | P01580 |
| Gene Symbol | IFNG |
| Entrez Gene ID | 15978 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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