Recombinant Mouse TNF-alpha Protein (EXRP010)
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For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
Key Features
✓ Endotoxin Level: <0.05 EU/1ug
✓ Purity: >95% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: E. coli
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Product Details
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Data Images
Background
Product Documents
Product Details
| Biological Activity | Determined by a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of actinomycin D. The ED₅₀ for this effect is typically 10-40 pg/mL. |
| Purity | >95% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.05 EU per 1 µg of the protein as determined by the LAL method. |
| Expression System | E. coli |
| Accession Number | P06804 |
| Sequence | Leu80-Leu235, with an N-terminal Met
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
| Molecular Weight | 17.4 kDa (predicted) |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
Data Images
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| Determined by a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of actinomycin D. The EDâ‚…â‚€ for this effect is typically 10 – 40 pg/mL. | Recombinant Mouse TNF-alpha (2 ug/lane) on SDS-AGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 17 kDa and purity greater than 95%. |
Background
| Alternative Names | Tumor Necrosis Factor alpha, TNF, TNFA, APC1, TNFSF2, Tumor Necrosis Factor Ligand Superfamily Member 2, Differentiation Inducing Factor, DIF, Cachectin |
| Function | Tumor Necrosis Factor-alpha (TNF-alpha) was initially associated as a proinflammatory cytokine expressed by macrophages. However, TNF-alpha also regulates apoptosis, tumor cell death, lipid metabolism and cell differentiation of T cells, B cells and NK cells. There are 2 receptors for TNF-alpha, a ubiquitously expressed TNFR1 (CD120a) homotrimer containing an apoptotic pathway trigger and the TNFR2 (CD120b) homotrimer that is commonly restricted to epithelial, endothelial and specific immune cells. |
| Tissue Specificity | TNF-alpha expression has been identified in adipose tissue, microglia, fibroblasts, endothelia tissue, and epithelial tissue. |
| Cellular Localization | Membrane-bound and cleaved soluble protein forms |
| Involvement in Disease | Dysregulation of TNF-alpha is implicated in rheumatoid arthritis, inflammatory bowel disease (IBD), psoriasis, ankylosing spondylitis, systemic lupus erythematosus (SLE), systemic sclerosis (SSc), multiple sclerosis (MS) and insulin resistance in type 2 diabetes. |
| UniProt | P06804 |
| Gene Symbol | TNF |
| Entrez Gene ID | 21926 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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