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For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
Key Features
✓ Detects endogenous PCNA
✓ Western blot, Immunohistochemistry, Immunofluorescence
✓ Monoclonal Mouse
Size
✓ 100 ug
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Source | Monoclonal Mouse |
| Specificity | Detects endogenous human, mouse, and rat PCNA. |
| Immunogen | PCNA synthetic peptide |
| Purification | Antigen affinity chromatography |
| Formulation | PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide as a preservative and 50% glycerol. |
| Concentration | 1 mg/mL |
| Applications | Western blot: 1:5,000 recommended dilution Immunofluorescence: 1:200 recommended dilution Immunohistochemistry: 1:200 recommended dilution For optimal performance, this reagent should be titrated for each application. |
Storage & Preparation
| Shipping | Shipped on blue ice. |
| Stability & Storage | 12 months from date of receipt at -15°C to -20°C. |
Data Images
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| Detection of PCNA by Western blot. Endogenous PCNA was detected at approximately 30-33 kDa in 1) HeLa cell lysates 2) rat brain tissue, 3) NIH 3T3 cell lysates, and 4) 293T cell lysates using Catalog # EXAB015 at 1:5,000 dilution. |
Background
| Alternative Names | Proliferating Cell Nuclear Antigen |
| Tissue Specificity | Bone marrow, Fetal brain cortex, Lung, Placenta |
| Cellular Localization | PCNA exists in normal proliferating cells and tumor cells, and locates in the nucleus. |
| UniProt | Human: P12004 Mouse: P17918 Rat: P04961 |
| Gene Symbol | PCNA |
| Entrez Gene ID | Human: 5111 Mouse: 18538 Rat: 25737 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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