Exreprotein uses cookies and similar technologies as strictly necessary to make our site work. We and our partners would also like to set additional cookies to analyze your use of our site, to personalize and enhance your visit to our site and to show you more relevant content and advertising. These will be set only if you accept. You can always review and change your cookie preferences through our cookie settings page. For more information, please read our Privacy Statement.
EXact-Cut™ EcoRI Restriction Endonuclease
Catalog: EXNA014
$49.00
Description
EXact-Cut EcoRI Restriction Endonuclease (EXNA014) cuts DNA at the G↓AATTC recognition sequence. As part of the EXact-Cut series, it works in a single universal buffer. This design then reduces star activity and speeds up digestion to 5–15 minutes. Furthermore, purple loading dye comes included for direct gel loading.
Key Features
✓ Reduced star activity
✓ Save time. Digest DNA in 5-15 minutes
✓ All Exact-Cut™ Enzymes 100% active in a single buffer (included)
✓ Purple Loading Dye included
Size
✓ 10,000 Units
Details
Order Review
EXact-Cut™ EcoRI Restriction Endonuclease
For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
Need Help Ordering?
Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Restriction Enzyme Site | 5ʹ…G↓AATT C…3ʹ 3ʹ…C TTAA↑G…5ʹ Isoschizomers: FunII (Isoschizomers may have different methylation sensitivities) |
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1 ug of λ DNA in 1 hour at 37°C in a total reaction volume of 50 uL. |
| Recommended Reaction Conditions | 1 x EXact-Cut™ Buffer Incubate at 37°C Refer to datasheet for reaction setup |
| Heat Inactivation | 1. Incubate at 80°C for 20 minutes 2. Add appropriate volume of 6X Gel Loading Dye, according to the reaction system |
| Product Components | EXact-Cut™ EcoRI (10 Units/uL): 2 x 5,000 Units EXact-Cut™ 10X Buffer: 6 x 1 mL 6X Gel Loading Dye, Purple: 1 mL |
Storage & Preparation
| Shipping | Shipped on blue ice. |
| Stability & Storage | Store at -20°C for up to 24 months. |
Data Images
Background
| Product Summary | EXact-Cut™ EcoRI Restriction Endonuclease is engineered for high specificity, reduced star activity, and time saving DNA digestion in 5-15 minutes. To simplify experimental design using multiple restriction enzymes, our entire range of EXact-Cut™ restriction endonucleases are 100% active in our EXact-Cut™ buffer (included) and are optimized for single-tube reactions along with digestion and ligation protocols. |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
Recommended Products
Reviews (no reviews yet)
Reviews
Overall User Score
0.0 /5
★★★★★
Based on 0 reviews
No reviews yet. Be the first to review this product.
Reviews
There are no reviews yet.