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EXact-Cut™ NcoI Restriction Endonuclease
Catalog: EXNA024
$49.00
Description
EXact-Cut NcoI Restriction Endonuclease (EXNA024) cuts DNA at the C↓CATGG recognition sequence, generating cohesive ends. As a result, its cut site overlaps the ATG start codon, making it widely used in expression vector cloning. Researchers therefore use it to insert coding sequences in-frame. Furthermore, it works in a single universal buffer with purple loading dye included.
Key Features
✓ Reduced star activity
✓ Save time. Digest DNA in 5-15 minutes
✓ All Exact-Cut™ Enzymes 100% active in a single buffer (included)
✓ Purple Loading Dye included
Size
✓ 500 Units
Details
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EXact-Cut™ NcoI Restriction Endonuclease
For Research Use Only.
Not Intended for Diagnostic or Therapeutic Use.
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Restriction Enzyme Site | 5ʹ…C↓CATG G…3ʹ 3ʹ…G GTAC↑C…5ʹ Isoschizomers: Bsp191 (Isoschizomers may have different methylation sensitivities) |
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1 ug of λ DNA in 1 hour at 37°C in a total reaction volume of 50 uL. |
| Recommended Reaction Conditions | 1 x EXact-Cut™ Buffer Incubate at 37°C Refer to datasheet for reaction setup |
| Heat Inactivation | 1. Incubate at 80°C for 20 minutes 2. Add appropriate volume of 6X Gel Loading Dye, according to the reaction system |
| Product Components | EXact-Cut™ NcoI (10 Units/uL): 500 Units EXact-Cut™ 10X Buffer: 1 mL 6X Gel Loading Dye, Purple: 1 mL |
Storage & Preparation
| Shipping | Shipped on blue ice. |
| Stability & Storage | Store at -20°C for up to 24 months. |
Data Images
Background
| Product Summary | EXact-Cut™ NcoI Restriction Endonuclease is engineered for high specificity, reduced star activity, and time saving DNA digestion in 5-15 minutes. To simplify experimental design using multiple restriction enzymes, our entire range of EXact-Cut™ restriction endonucleases are 100% active in our EXact-Cut™ buffer (included) and are optimized for single-tube reactions along with digestion and ligation protocols. |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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