Recombinant Bovine Serum Albumin/BSA Protein (EXRP053)
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Key Features
✓ Endotoxin Level: <0.001 EU/1ug (<1 EU/mg)
✓ Purity: >98% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: CHO cell line, with no Fetal Bovine Serum (FBS)
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Biological Activity | The carrier effect of Recombinant Bovine Serum Albumin/BSA was determined by enhancement of cell proliferation using TF-1 human cells. Stout, et al. (2022) Commun Biol 5:466. Cell proliferation in TF-1 human cells was induced in a dose-dependent manner by either Recombinant Human GM-CSF or Recombinant Human IL-3 (Catalog # EXRP037) and demonstrated >25% enhancement in the presence of rBSA. |
| Purity | >98% under reducing conditions by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.001 EU/1ug (<1 EU/mg) of the protein as determined by the LAL method. |
| Expression System | CHO cell line, with no Fetal Bovine Serum (FBS). |
| Accession Number | A0A140T897 |
| Sequence | Asp25-Ala607
DTHKSEIAHR FKDLGEEHFK GLVLIAFSQY LQQCPFDEHV KLVNELTEFA KTCVADESHA GCEKSLHTLF GDELCKVASL RETYGDMADC CEKQEPERNE CFLSHKDDSP DLPKLKPDPN TLCDEFKADE KKFWGKYLYE IARRHPYFYA PELLYYANKY NGVFQECCQA EDKGACLLPK IETMREKVLT SSARQRLRCA SIQKFGERAL KAWSVARLSQ KFPKAEFVEV TKLVTDLTKV HKECCHGDLL ECADDRADLA KYICDNQDTI SSKLKECCDK PLLEKSHCIA EVEKDAIPEN LPPLTADFAE DKDVCKNYQE AKDAFLGSFL YEYSRRHPEY AVSVLLRL AK EYEATLEECC AKDDPHACYS TVFDKLKHLV DEPQNLIKQN CDQFEKLGEY GFQNALIVRY TRKVPQVSTP TLVEVSRSLG KVGTRCCTKP ESERMPCTED YLSLILNRLC VLHEKTPVSE KVTKCCTESL VNRRPCFSAL TPDETYVPKA FDEKLFTFHA DICTLPDTEK QIKKQTALVE LLKHKPKATE EQLKTVMENF VAFVDKCCAA DDKEACFAVE GPKLVVSTQT ALA |
| Molecular Weight | 66.5 kDa (monomer, predicted), 81 kDa (glycosylated, observed on SDS-PAGE gel stained with stained using Coomassie® Blue under reducing conditions) |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 1 mg/mL in sterile PBS. |
Data Images
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| Recombinant Bovine Serum Albumin/BSA Protein (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 81 kDa due to glycosylation and purity greater than 98%. Identity confirmed by polyclonal anti-BSA antibody in Western blot. | Carrier effect of rBSA is observed in the cell proliferation in TF-1 human cells treated with Recombinant Human GM-CSF in the presence of 50 ug/mL rBSA. The carrier effect due to rBSA does not alter the ED50 in this assay. |
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| Carrier effect of rBSA is observed in the cell proliferation in TF-1 human cells treated with Recombinant Human IL-3 (Catalog # EXRP037) in the presence of 25 ug/mL rBSA. The carrier effect due to rBSA does not alter the ED50 in this assay. |
Background
| Alternative Names | Albumin, BSA |
| Function | Bovine serum albumin (BSA) is an approximately 66.5 kDa protein with diverse functions including maintenance of pH and osmotic balance in biochemical assays and culture systems, along with functioning as a carrier protein that binds and transports a wide range of small molecules, including fatty acids, hormones, bilirubin, and metal ions. Additionally, BSA functions as a stabilizer of proteins and enzymes with properties that limit denaturation, aggregation and proteolysis. Due to these functions, recombinant BSA restores many of the transport, protective, and buffering roles lost when serum is removed from chemically defined cell culture media. |
| Tissue Specificity | Plasma |
| Cellular Localization | Secreted protein |
| UniProt | A0A140T897 |
| Gene Symbol | ALB |
| Entrez Gene ID | 280717 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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